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Thermo Fisher amplex red cholesterol assay kit
Differences in <t>cholesterol</t> removal capacity and cellular association of sHDL particles. (A) Washed human platelets were incubated with 22A peptide (100 μg/ml) and different sHDL particles (100 μg/ml of 22A peptide) for 15 min. The total cholesterol was quantified via Amplex Red Cholesterol Assay Kit. (B–E) Cellular associations of DiI-labeled sHDL particles (sHDLs at 100 μg/ml 22A, 200 μg/ml lipid, and 1 μg/ml DiI) following 5, 15, 30, and 60 min incubations with human washed platelets. Mean fluorescence intensity and representative histogram of DiI in platelets. Data represent mean ± SD (n = 5); ns = not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 versus control; #### P < 0.0001 versus 22A:POPC; §§ P < 0.01, §§§ P < 0.001, §§§§ P < 0.0001 versus 22A:DMPC.
Amplex Red Cholesterol Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Differences in cholesterol removal capacity and cellular association of sHDL particles. (A) Washed human platelets were incubated with 22A peptide (100 μg/ml) and different sHDL particles (100 μg/ml of 22A peptide) for 15 min. The total cholesterol was quantified via Amplex Red Cholesterol Assay Kit. (B–E) Cellular associations of DiI-labeled sHDL particles (sHDLs at 100 μg/ml 22A, 200 μg/ml lipid, and 1 μg/ml DiI) following 5, 15, 30, and 60 min incubations with human washed platelets. Mean fluorescence intensity and representative histogram of DiI in platelets. Data represent mean ± SD (n = 5); ns = not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 versus control; #### P < 0.0001 versus 22A:POPC; §§ P < 0.01, §§§ P < 0.001, §§§§ P < 0.0001 versus 22A:DMPC.

Journal: Journal of Lipid Research

Article Title: Phospholipase A 2 products influence the antiplatelet functions of synthetic high-density lipoproteins

doi: 10.1016/j.jlr.2025.100972

Figure Lengend Snippet: Differences in cholesterol removal capacity and cellular association of sHDL particles. (A) Washed human platelets were incubated with 22A peptide (100 μg/ml) and different sHDL particles (100 μg/ml of 22A peptide) for 15 min. The total cholesterol was quantified via Amplex Red Cholesterol Assay Kit. (B–E) Cellular associations of DiI-labeled sHDL particles (sHDLs at 100 μg/ml 22A, 200 μg/ml lipid, and 1 μg/ml DiI) following 5, 15, 30, and 60 min incubations with human washed platelets. Mean fluorescence intensity and representative histogram of DiI in platelets. Data represent mean ± SD (n = 5); ns = not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 versus control; #### P < 0.0001 versus 22A:POPC; §§ P < 0.01, §§§ P < 0.001, §§§§ P < 0.0001 versus 22A:DMPC.

Article Snippet: The total cholesterol was determined using the Amplex Red Cholesterol Assay Kit (Invitrogen, ThermoFisher Scientific, Waltham, MA) according to the manufacturer’s procedures.

Techniques: Incubation, Amplex Red Cholesterol Assay, Labeling, Fluorescence, Control